9HRD

Crystal structure of the Class V GTP aptamer in complex with GTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-09-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.892
Spacegroup name	P 1 21 1
Unit cell lengths	52.268, 127.216, 68.368
Unit cell angles	90.00, 90.45, 90.00

Refinement procedure

Resolution	20.001 - 2.500
Rwork	0.200
R-free	0.23780
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.873
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.001	2.590
High resolution limit [Å]	2.500	2.500
Rmerge	0.090
Rpim	0.037	0.877
Number of reflections	30103	2966
<I/σ(I)>	11.23	0.75
Completeness [%]	97.3
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	291	methylpentanediol, potasium chloride, manganese chloride, spermine, cacodylate