9HQ8

Hypothetical protein from ssRNA Leviviricetes sp bacteriophage metagenome, IMGVR_UViG_3300036404_000292

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2024-10-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9184
Spacegroup name	I 21 21 21
Unit cell lengths	54.440, 61.290, 122.610
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.700 - 2.000
R-factor	0.23728
Rwork	0.235
R-free	0.30670
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.705
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.700	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.175
Number of reflections	16564	1062
<I/σ(I)>	1.7
Completeness [%]	100.0
Redundancy	12.8
CC(1/2)	0.999	0.690

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	1.4 M ammonium sulfate, 0.1 M MES pH = 6.5, 10% 1,4-dioxane