9HPS

Human BclxLdeltaLT-VDAC1-N fusion protein complex structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-09-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.99999
Spacegroup name	C 2 2 21
Unit cell lengths	35.120, 98.750, 103.910
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.640 - 1.950
R-factor	0.17135
Rwork	0.170
R-free	0.20055
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.759
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.000
High resolution limit [Å]	1.950	1.950
Number of reflections	12735	1012
<I/σ(I)>	12.6	2.3
Completeness [%]	98.7
Redundancy	4.1
CC(1/2)	0.997	0.802

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	9	292	1.6 M ammonium sulfate, 0.1 M bicine pH 9