9HPB

Mouse phosphomannomutase 2 in apo state from crystals with PEG 8000

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	80.657, 98.611, 212.849
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	53.210 - 2.430
R-factor	0.2359
Rwork	0.234
R-free	0.27050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.622
Data reduction software	XDS (1.20.1_4487)
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1-4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.430	2.480
High resolution limit [Å]	2.430	2.430
Rpim	0.046	0.873
Number of reflections	64543	3193
<I/σ(I)>	10.6	0.9
Completeness [%]	99.9	99.9
Redundancy	6.6
CC(1/2)	0.999	0.614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		294	Best crystals appeared after 2-7 days using 0.1 M Tris-HCl pH 8.5, 0.2 M MgCl2, and 20-22% PEG 8000 as reservoir, in hanging drop 24-well plates, by mixing 2 ul of protein with 2 ul of reservoir solution