9HO6

Crystal Structure of the Human Frataxin protein in complex with a tailored Camelid Nanobody 16C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-20
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97926
Spacegroup name	P 21 21 21
Unit cell lengths	57.899, 81.100, 107.546
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	64.750 - 2.000
R-factor	0.1824
Rwork	0.180
R-free	0.22740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.996
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21rc1_4958)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.752	2.183
High resolution limit [Å]	1.997	1.997
Number of reflections	25980	1299
<I/σ(I)>	14.2
Completeness [%]	91.7
Redundancy	11.5
CC(1/2)	0.997	0.390

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	15% PEG 10K 0.1M Ammonium acetate 0.1M Bis-Tris pH 5.5