9HO4
Crystal Structure of the Human Frataxin protein in complex with a tailored Camelid Nanobody 6B1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-09-16 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97926 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 97.253, 97.253, 128.105 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 84.224 - 1.760 |
| Rwork | 0.151 |
| R-free | 0.17530 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.362 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430 (refmacat 0.4.105)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.224 | 1.584 |
| High resolution limit [Å] | 1.480 | 1.485 |
| Number of reflections | 50815 | 2541 |
| <I/σ(I)> | 33.5 | |
| Completeness [%] | 96.8 | 74.5 |
| Redundancy | 20.5 | 21.7 |
| CC(1/2) | 0.999 | 0.571 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1M HEPES pH 7.0 0.8M Potassium Sodium Tartrate |
