9HNV

Crystal structure of a N-terminal GH19 domain of DS6A-LysA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9792
Spacegroup name	I 2 2 2
Unit cell lengths	89.175, 145.941, 147.703
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	76.340 - 2.750
R-factor	0.241
Rwork	0.239
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.614
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	76.340	2.880
High resolution limit [Å]	2.750	2.750
Rpim	0.128	0.799
Number of reflections	25458	3356
<I/σ(I)>	6.7
Completeness [%]	100.0
Redundancy	8.3
CC(1/2)	0.984	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	2.2M de NaCl, 50 mM Tris-HCl pH 8