9HNK
Structure of the (6-4) photolyase of Caulobacter crescentus in its oxidizd state - Singal crystal / Synchrotron
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-10 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.274, 102.730, 103.893 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.360 - 1.590 |
| R-factor | 0.1821 |
| Rwork | 0.181 |
| R-free | 0.20160 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.232 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.360 | 1.650 |
| High resolution limit [Å] | 1.590 | 1.590 |
| Rmerge | 0.114 | 1.749 |
| Number of reflections | 737710 | 7104 |
| <I/σ(I)> | 8.3 | 1.41 |
| Completeness [%] | 99.5 | |
| Redundancy | 10.2 | |
| CC(1/2) | 0.997 | 0.520 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 278 | 1.0M LiCl 0.1M MES (pH 6.0) 20% (w/v) PEG6000 |
