9HNA
Crystal structure of apo Ami2B domain of DS6A-LysA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-11 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9793 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 71.998, 121.085, 80.731 |
| Unit cell angles | 90.00, 107.16, 90.00 |
Refinement procedure
| Resolution | 77.140 - 2.040 |
| R-factor | 0.1869 |
| Rwork | 0.186 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond angle | 0.788 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.20.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.140 | 2.071 |
| High resolution limit [Å] | 2.036 | 2.036 |
| Rmerge | 0.134 | 1.240 |
| Rpim | 0.055 | 0.497 |
| Number of reflections | 41716 | 2098 |
| <I/σ(I)> | 10.2 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.8 | 7.1 |
| CC(1/2) | 0.994 | 0.666 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M HEPES pH 7.5, 0.8M sodium phosphate y 0.4M de potassium phosphate |
