9HN5
Cryo-EM structure of human separase bound to phosphorylated SCC1 (100-320 aa)
EM specimen
| Vitrification | Yes |
| Instrument | LEICA EM GP |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | TFS Selectris X |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.96 |
| Number of Particle | 12609660 |
| Software | cryoSPARC,Coot,EPU,UCSF ChimeraX,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
