9HMB
Crystal structure of GH139 glycoside hydrolase from Verrucomicrobium sp. in the hexagonal space group P6522
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 209.450, 209.450, 143.960 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 76.740 - 2.050 |
| R-factor | 0.1347 |
| Rwork | 0.133 |
| R-free | 0.17190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.953 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 76.740 | 2.123 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Rmerge | 0.050 | |
| Rmeas | 0.789 | |
| Number of reflections | 222324 | 5422 |
| <I/σ(I)> | 71.7 | |
| Completeness [%] | 100.0 | |
| Redundancy | 39.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | 0.2 M Sodium citrate tribasic dihydrate, 0.1 M Bis-Tris propane pH 8.5 and 20 % w/v PEG 3350 |
