9HK9

Crystal structure of IMPDH from Burkholderia thailandensis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-04-21
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979337
Spacegroup name	I 4
Unit cell lengths	118.960, 118.960, 141.840
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.570 - 1.860
R-factor	0.1776
Rwork	0.176
R-free	0.21490
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.771
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.570	1.928
High resolution limit [Å]	1.860	1.862
Number of reflections	82117	8091
<I/σ(I)>	18
Completeness [%]	99.8
Redundancy	14.1
CC(1/2)	0.999	0.743

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	20%w/v PEG 3350; 150 mM Na Malonate