9HK1
PD1 signaling receptor bound to FAB Complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-17 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 1 |
| Unit cell lengths | 52.161, 53.794, 103.003 |
| Unit cell angles | 104.80, 101.83, 92.57 |
Refinement procedure
| Resolution | 51.848 - 2.030 |
| Rwork | 0.210 |
| R-free | 0.27550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.785 |
| Data reduction software | DIALS |
| Data scaling software | DIALS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.850 | 2.060 |
| High resolution limit [Å] | 2.030 | 2.030 |
| Rmerge | 0.132 | 1.286 |
| Number of reflections | 67042 | 6591 |
| <I/σ(I)> | 11.11 | 1.07 |
| Completeness [%] | 98.0 | 95.11 |
| Redundancy | 3.63 | 3.71 |
| CC(1/2) | 0.992 | 0.222 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 20.5% PEG 3350, 0.4M MgCl2, 0.1M Bis-Tris pH 5.5 |
