9HK0

PNT domain of human ETV7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 11.2C
Synchrotron site	ELETTRA
Beamline	11.2C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-11-22
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00
Spacegroup name	P 64
Unit cell lengths	67.813, 67.813, 29.831
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	33.907 - 1.610
R-factor	0.1847
Rwork	0.184
R-free	0.20500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.792
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.730	1.640
High resolution limit [Å]	1.610	1.610
Rmerge	0.051	1.723
Rmeas	0.053	1.769
Rpim	0.012	0.397
Total number of observations	192678	9585
Number of reflections	10339	490
<I/σ(I)>	28.4	1.9
Completeness [%]	100.0
Redundancy	18.6	19.6
CC(1/2)	1.000	0.845

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	20% PEG3350