9HJ5

Engineered Fructosyl Peptide Oxidase - D7 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-19
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 1
Unit cell lengths	54.083, 54.120, 82.657
Unit cell angles	80.07, 85.53, 89.84

Refinement procedure

Resolution	53.974 - 2.093
Rwork	0.193
R-free	0.25710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.519
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	REFMAC
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	53.974	2.129
High resolution limit [Å]	2.093	2.093
Number of reflections	52616	2633
<I/σ(I)>	5.2
Completeness [%]	96.9
Redundancy	3.2
CC(1/2)	0.982	0.357

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	277.15	12% PEG 8K, 5% Ethanol, 0.1M Tris-HCl pH 8.0