9HIB
K115 acetylated human muscle pyruvate kinase, isoform M2 (PKM2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-02-19 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.536, 154.783, 100.700 |
| Unit cell angles | 90.00, 107.40, 90.00 |
Refinement procedure
| Resolution | 81.640 - 2.170 |
| R-factor | 0.22505 |
| Rwork | 0.223 |
| R-free | 0.26503 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 2.142 |
| Data reduction software | xia2 |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 81.640 | 2.248 |
| High resolution limit [Å] | 2.170 | 2.170 |
| Number of reflections | 125584 | 12530 |
| <I/σ(I)> | 12.9 | |
| Completeness [%] | 99.8 | |
| Redundancy | 7.1 | |
| CC(1/2) | 0.973 | 0.347 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 5% Tacsimate 0.1M Hepes pH 6.8 12.14% PEG MME 5000 |
