9HI1

STRUCTURE OF HUMAN UDP-GALACTOSE 4-EPIMERASE IN COMPLEX WITH COMPOUND WBX09

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-02
Detector	DECTRIS EIGER2 S 16M
Wavelength(s)	0.7098
Spacegroup name	P 21 21 21
Unit cell lengths	46.147, 110.205, 135.411
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.755 - 0.950
Rwork	0.181
R-free	0.18900
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	2.105
Data reduction software	DIALS
Data scaling software	xia2.multiplex
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.88))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.760	0.970
High resolution limit [Å]	0.950	0.950
Number of reflections	415408	22430
<I/σ(I)>	76.5
Completeness [%]	99.4
Redundancy	40
CC(1/2)	1.000	0.330

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	18% PEG 3350 0.2 M Na Malonate