Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-04-20 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00003 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 93.030, 73.220, 52.360 |
| Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
| Resolution | 57.490 - 2.750 |
| R-factor | 0.2031 |
| Rwork | 0.201 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.250 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.490 | 2.900 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.152 | 1.270 |
| Rpim | 0.109 | 0.750 |
| Number of reflections | 9186 | 1474 |
| <I/σ(I)> | 5.8 | 0.9 |
| Completeness [%] | 99.7 | 98.5 |
| Redundancy | 3.1 | 3 |
| CC(1/2) | 0.994 | 0.586 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 293 | 0.2M CaCl2, 0.1 M Mops pH 6.2, 11-14% PEG8000, after microseeding |
