9HGF
Crystal Structure of the Plasmodium vivax Bromodomain PvBDP1 in complex with RMM25
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-3 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-3 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-01-09 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 0.967697 |
| Spacegroup name | P 62 2 2 |
| Unit cell lengths | 92.798, 92.798, 70.721 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.090 - 1.860 |
| R-factor | 0.2059 |
| Rwork | 0.204 |
| R-free | 0.23440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.067 |
| Data reduction software | autoPROC |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21_5207) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.365 | 1.891 |
| High resolution limit [Å] | 1.859 | 1.859 |
| Rmerge | 0.134 | 2.255 |
| Rmeas | 0.136 | 2.284 |
| Rpim | 0.022 | 0.361 |
| Number of reflections | 15625 | 762 |
| <I/σ(I)> | 20.6 | 2.4 |
| Completeness [%] | 99.9 | |
| Redundancy | 37.3 | 39.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 35% PEG 3350, 0.2M LiSO4, Tris pH 7.5 |
