9HFU
Caprin1 peptide bound to SPOP MATH domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-10 |
| Detector | DECTRIS EIGER2 X CdTe 9M |
| Wavelength(s) | 0.6199 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.808, 60.882, 57.117 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 41.660 - 1.700 |
| R-factor | 0.1646 |
| Rwork | 0.162 |
| R-free | 0.20390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.027 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.670 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 29981 | 2918 |
| <I/σ(I)> | 6.9 | |
| Completeness [%] | 99.5 | |
| Redundancy | 1.9 | |
| CC(1/2) | 0.990 | 0.850 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 12.5% w/v PEG 1000, 12.5% w/v PEG 3350, 12.5% v/v MPD, 0.03 M sodium fluoride, 0.03 M sodium bromide, 0.03 M sodium iodide, 0.1 M bicine/Trizma base pH 8.5 |
