9HEV
CryoEM structure of mouse Panx1 phosphorylated in vitro by Src, narrow conformation
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.93 |
| Number of Particle | 745409 |
| Software | Topaz,cryoSPARC,EPU,ISOLDE,PHENIX |
| Symmetry | C7 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
