Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-01-29 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 92.178, 92.178, 142.973 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.255 - 1.510 |
| Rwork | 0.184 |
| R-free | 0.21490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.555 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.255 | 1.540 |
| High resolution limit [Å] | 1.510 | 1.510 |
| Rmerge | 0.095 | 4.399 |
| Rmeas | 0.097 | 4.559 |
| Rpim | 0.022 | 1.178 |
| Number of reflections | 110596 | 5481 |
| <I/σ(I)> | 14.5 | 0.3 |
| Completeness [%] | 100.0 | |
| Redundancy | 20.1 | 14.6 |
| CC(1/2) | 1.000 | 0.324 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 3.0 M Ammonium sulfate; 1% (w/v) MPD |
