9HAX
F420-dependent glucose-6-phosphate dehydrogenase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-20 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 104.528, 239.408, 108.200 |
| Unit cell angles | 90.00, 108.59, 90.00 |
Refinement procedure
| Resolution | 49.234 - 2.513 |
| Rwork | 0.231 |
| R-free | 0.25710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.672 |
| Data reduction software | XDS (Feb 5, 2021) |
| Data scaling software | XDS (Feb 5, 2021) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0425) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.670 |
| High resolution limit [Å] | 2.510 | 2.510 |
| Number of reflections | 167400 | 25908 |
| <I/σ(I)> | 4.9 | 0.8 |
| Completeness [%] | 98.0 | 94.1 |
| Redundancy | 6.4 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 12% PEG4000, 0.1 M NaOAc pH 4.6, 0.2 M (NH4)2SO4 |
