9HAW

F420-dependent glucose-6-phosphate dehydrogenase without ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-08-11
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9762
Spacegroup name	P 21 21 2
Unit cell lengths	200.482, 376.876, 104.772
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	177.626 - 2.220
Rwork	0.173
R-free	0.19330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.505
Data reduction software	XDS (Jan 31, 2020)
Data scaling software	XDS (Jan 31, 2020)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	180.000	2.320
High resolution limit [Å]	2.220	2.220
Number of reflections	351453	17574
<I/σ(I)>	10.2	1.5
Completeness [%]	90.3	38.6
Redundancy	4.2	4.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M NH4F, 20% PEG3350