9H9C
Crystal structure of thioredoxin reductase from Cryptosporidium parvum in the "activated in" conformation
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.872, 72.543, 188.458 |
| Unit cell angles | 90.00, 90.61, 90.00 |
Refinement procedure
| Resolution | 39.910 - 2.400 |
| Rwork | 0.223 |
| R-free | 0.25680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.533 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.910 | 2.486 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.120 | 0.780 |
| Number of reflections | 78143 | 7744 |
| <I/σ(I)> | 12.72 | |
| Completeness [%] | 99.5 | |
| Redundancy | 6.5 | |
| CC(1/2) | 0.996 | 0.790 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294.15 | 0.1 M Tris/HCl, 0.1 M KSCN, PEG 2000 MME, 5 mM DTT |
