9H8Q
Eugenol Oxidase (EUGO) from Rhodococcus jostii RHA1, mutant DTT-T425G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-25 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.9655 |
| Spacegroup name | P 1 |
| Unit cell lengths | 90.439, 110.197, 117.536 |
| Unit cell angles | 89.87, 89.34, 68.51 |
Refinement procedure
| Resolution | 102.750 - 1.600 |
| R-factor | 0.1783 |
| Rwork | 0.177 |
| R-free | 0.19774 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.604 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 117.530 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.073 | 0.906 |
| Rmeas | 0.090 | 1.132 |
| Rpim | 0.052 | 0.669 |
| Total number of observations | 1437509 | 41900 |
| Number of reflections | 519315 | 16604 |
| <I/σ(I)> | 9.7 | 1.3 |
| Completeness [%] | 93.2 | |
| Redundancy | 2.8 | 2.5 |
| CC(1/2) | 0.995 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.8 | 293 | 41-46% MPD and 0.1 M bis-tris propane |
