9H6S
Structure of the V606M/W622L mutant of myosin UNC-54
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-09-25 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.885603 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.033, 124.272, 96.957 |
| Unit cell angles | 90.00, 109.88, 90.00 |
Refinement procedure
| Resolution | 76.780 - 2.400 |
| R-factor | 0.2133 |
| Rwork | 0.212 |
| R-free | 0.24010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.648 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 77.000 | 2.432 | 2.196 |
| High resolution limit [Å] | 2.159 | 2.376 | 2.159 |
| Number of reflections | 103942 | 5410 | 5384 |
| <I/σ(I)> | 2.64 | ||
| Completeness [%] | 95.6 | ||
| Redundancy | 3.64 | ||
| CC(1/2) | 0.986 | 0.377 | 0.127 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% (w/v) PEG335, 200 mM sodium formate |
