9H6E

Complex of Histidine-containing phosphotransfer 1 (AHP1) and Response regulator 1 (ARR1) from A. thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9196
Spacegroup name	P 21 21 21
Unit cell lengths	84.500, 132.574, 160.672
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.250 - 2.870
R-factor	0.1926
Rwork	0.191
R-free	0.22860
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.140
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.336	3.150
High resolution limit [Å]	2.870	2.874
Rmerge	0.110	0.920
Number of reflections	30328	1517
<I/σ(I)>	15.5	3.2
Completeness [%]	72.4	15.3
Redundancy	13.6	14.1
CC(1/2)	0.998	0.810

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	292	20 mM Sodium formate, 20 mM ammonium acetate, 20 sodium citrate tribasic dihydrate, 20 mM potassium sodium tartrate tetrahydrate, 20 mM sodium oxamate, 100 mM Tris (base) BICINE pH 8.5, 20% glycerol, 10% PEG 4000