9H6D

Crystal structure of the E. coli F-plasmid VapBC toxin-antitoxin complex (VapB V5E)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-28
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.977020
Spacegroup name	P 32 2 1
Unit cell lengths	89.807, 89.807, 117.134
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.790 - 3.150
R-factor	0.2509
Rwork	0.248
R-free	0.29840
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.679
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.20.1_4487: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.800	46.800	3.230
High resolution limit [Å]	3.150	14.080	3.150
Rmerge	0.117	0.023	4.692
Rmeas	0.123	0.025	4.937
Number of reflections	9854	130	707
<I/σ(I)>	13.84
Completeness [%]	99.8
Redundancy	20.08
CC(1/2)	0.999	1.000	0.167

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	0.2 M Lithium sulphate monohydrate, 0.1 M sodium citrate tribasic dihydrate, 26 % PEG200