9H4X

Crystal structure of Ni2+ dependent glycerol-1-phosphate dehydrogenase AraM from Bacillus subtilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-09-28
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9677
Spacegroup name	C 1 2 1
Unit cell lengths	92.015, 70.499, 72.609
Unit cell angles	90.00, 95.51, 90.00

Refinement procedure

Resolution	43.036 - 2.950
Rwork	0.241
R-free	0.31020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.509
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0425)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.510	3.130
High resolution limit [Å]	2.950	2.950
Number of reflections	9676	1527
<I/σ(I)>	8
Completeness [%]	98.4
Redundancy	1.9
CC(1/2)	0.978	0.433

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8.2	293	20.0-25.5% (w/v) PEG 3350, 0.15-0.29 M KSCN