9H49
Crystal structure of the adduct between human serum transferrin (apo-form) and cisplatin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-02-04 |
| Detector | DECTRIS EIGER2 X CdTe 16M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 88.184, 103.680, 200.283 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 80.840 - 3.520 |
| R-factor | 0.23152 |
| Rwork | 0.231 |
| R-free | 0.24936 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.936 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.840 | 3.580 |
| High resolution limit [Å] | 3.520 | 3.520 |
| Number of reflections | 23356 | 1147 |
| <I/σ(I)> | 7.6 | 0.8 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.9 | 11.3 |
| CC(1/2) | 1.000 | 0.400 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% w/v PEG 3350 and 200 mM ammonium citrate pH 7.0 |
