9H42

EGFR wild type incomplex with 26007

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	173
Detector technology	PIXEL
Collection date	2023-12-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.92
Spacegroup name	I 2 3
Unit cell lengths	144.890, 144.890, 144.890
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.860 - 2.601
Rwork	0.184
R-free	0.24470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.899
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0430 (refmacat 0.4.82))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.860	2.720
High resolution limit [Å]	2.600	2.600
Rmerge	0.041	6.600
Number of reflections	15709	1903
<I/σ(I)>	19.2
Completeness [%]	100.0	100
Redundancy	40.5	42.2
CC(1/2)	1.000	0.350

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.6	293	25 mM HEPES pH 8, 150 mM NaCl, 10 % glycerol, 1 mM DTT 85mM Tris pH 7.6, 140 mM acetate, 23.5 % Polyethylene glycol 4,000, 18% glycerol