Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-02-27 |
| Detector | DECTRIS EIGER2 X 9M |
| Wavelength(s) | 0.96863 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 39.760, 103.648, 41.868 |
| Unit cell angles | 90.00, 104.42, 90.00 |
Refinement procedure
| Resolution | 40.550 - 1.280 |
| R-factor | 0.1988 |
| Rwork | 0.198 |
| R-free | 0.22080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.767 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.1_5286) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.550 | 1.350 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Number of reflections | 150574 | 22160 |
| <I/σ(I)> | 8.75 | |
| Completeness [%] | 90.2 | |
| Redundancy | 1.56 | |
| CC(1/2) | 0.999 | 0.472 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5 |
