9H2U

Crystal structure of YTHDC1 in complex with AI_033

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 1 21 1
Unit cell lengths	39.930, 103.750, 42.230
Unit cell angles	90.00, 104.84, 90.00

Refinement procedure

Resolution	40.820 - 1.210
R-factor	0.2004
Rwork	0.199
R-free	0.21890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.789
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.821	1.280
High resolution limit [Å]	1.210	1.210
Number of reflections	98597	15499
<I/σ(I)>	15.26
Completeness [%]	96.8
Redundancy	3.35
CC(1/2)	0.997	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	25% PEG 3350, 0.2M Ammonium Sulphate, 0.1M Bis-Tris pH 6.5