9H2N
Crystal structure of tyrosinase from Priestia megaterium F227Y mutant soaked in Cu(II) and sodium dithionite
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2023-04-02 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.8731 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.514, 78.039, 150.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.040 - 2.000 |
| R-factor | 0.2228 |
| Rwork | 0.219 |
| R-free | 0.28500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.912 |
| Phasing software | PHENIX (1.19_4092) |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 75.040 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 38439 | 3741 |
| <I/σ(I)> | 5.6 | 1.1 |
| Completeness [%] | 99.6 | 98.5 |
| Redundancy | 1.9 | 1.9 |
| CC(1/2) | 0.997 | 0.710 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 295.15 | 0.1 M magnesium formate dihydrate, 15% PEG 3500 |
