9H1W

Grouped 150-240 ms dark structure of sensory rhodopsin-II solved by serial millisecond crystallography

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	296
Detector technology	PIXEL
Collection date	2019-11-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000342
Spacegroup name	C 2 2 21
Unit cell lengths	89.750, 131.750, 51.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.880 - 2.200
R-factor	0.2158
Rwork	0.216
R-free	0.21850
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.099
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.880	44.880
High resolution limit [Å]	2.200	2.200
Number of reflections	4429	4429
<I/σ(I)>	22.88
Completeness [%]	100.0
Redundancy	268.03
CC(1/2)	0.994	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE		296	CaCl2 150 mM, Glycine 100 mM, 38% (v/v) PEG 400, pH 7.5