9H1K

RlmR 23S rRNA methyltransferase from Thermus thermophilus in complex with rRNA and S-adenosyl-L-homocysteine (SAH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-09-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9786
Spacegroup name	P 21 21 2
Unit cell lengths	113.040, 117.430, 58.250
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.352 - 1.895
R-factor	0.205115277933
Rwork	0.204
R-free	0.23257
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.011
RMSD bond angle	1.529
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.11_2563)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	58.715	58.715	1.928
High resolution limit [Å]	1.890	5.143	1.895
Number of reflections	62387	3361	3095
<I/σ(I)>	16
Completeness [%]	100.0
Redundancy	13.6
CC(1/2)	0.999	1.000	0.391

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	4.5	292	Na-Citrate 0.1M, pH5.5, PEG6000 10%, LiCl 1M