9H1J

Crystal structure of the p62 UBA domain bound to VHH 6C10

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-03-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00007
Spacegroup name	P 1 21 1
Unit cell lengths	62.886, 73.613, 89.785
Unit cell angles	90.00, 90.22, 90.00

Refinement procedure

Resolution	19.660 - 1.730
R-factor	0.2252
Rwork	0.214
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.368
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.890	1.760
High resolution limit [Å]	1.730	1.730
Rpim	0.040	1.337
Number of reflections	84831	4196
<I/σ(I)>	7.2
Completeness [%]	99.0
Redundancy	5.3
CC(1/2)	0.997	0.379

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1M Lithium Chloride, 0.1M Citric Acid, 20% (w/v) PEG6000