9H11
Crystal structure of OXA-48 apoenzyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-11 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.8622 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 123.513, 123.513, 161.564 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.760 - 1.380 |
| R-factor | 0.1709 |
| Rwork | 0.170 |
| R-free | 0.18690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.782 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.760 | 1.400 |
| High resolution limit [Å] | 1.380 | 1.380 |
| Rpim | 0.055 | 1.020 |
| Number of reflections | 148214 | 14595 |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 40.2 | 41.1 |
| CC(1/2) | 0.999 | 0.309 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 283 | 0.1 M HEPES pH 7.5, 33% v/v PEG 400 |
