Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-12-01 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 57.450, 57.450, 114.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 51.386 - 1.450 |
| R-factor | 0.1697 |
| Rwork | 0.168 |
| R-free | 0.19540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4o8h |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.851 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 51.390 | 1.489 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.063 | 0.409 |
| Rpim | 0.117 | |
| Number of reflections | 34902 | 2468 |
| <I/σ(I)> | 15.9 | 4.8 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 13.1 | 13.1 |
| CC(1/2) | 0.999 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 298 | 0.17 M Sodium acetate trihydrate,0.085 M Sodium cacodylate trihydrate pH 6.5,25.5% w/v Polyethylene glycol 8,000 |
