9GYP
Crystal structure of human Histidine Triad Nucleotide-Binding Protein 1 in complex with KV24
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | RIGAKU PhotonJet-S |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-12-14 |
| Detector | RIGAKU HyPix-6000HE |
| Wavelength(s) | 1.54184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.957, 46.446, 63.788 |
| Unit cell angles | 90.00, 94.53, 90.00 |
Refinement procedure
| Resolution | 18.416 - 1.801 |
| Rwork | 0.141 |
| R-free | 0.19080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.651 |
| Data reduction software | CrysalisPro (43.100a) |
| Data scaling software | Aimless (0.7.13) |
| Phasing software | MOLREP (11.9.02) |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.416 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.344 |
| Rmeas | 0.065 | 0.428 |
| Rpim | 0.032 | 0.251 |
| Number of reflections | 21094 | 1233 |
| <I/σ(I)> | 15.1 | 2.9 |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 3.8 | 2.4 |
| CC(1/2) | 0.998 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 281 | 10% w/v PEG4000, 0.1 M sodium cacodylate pH 6.0 |
