Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-08-07 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.62 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.286, 51.285, 61.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.321 - 1.000 |
| Rwork | 0.141 |
| R-free | 0.15610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.322 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.300 | 1.030 |
| High resolution limit [Å] | 1.000 | 1.000 |
| Rmerge | 0.081 | 2.500 |
| Rpim | 0.017 | 0.660 |
| Number of reflections | 52421 | 2565 |
| <I/σ(I)> | 15.7 | 1.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 24.4 | 15.4 |
| CC(1/2) | 0.999 | 0.672 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | 100 mM Na, K phosphate, 2.6-3.6 M Ammonium phosphate |
