9GY2
Non-Hemolytic Phospholipase C (PLC N)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-13 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.270, 110.432, 98.619 |
| Unit cell angles | 90.00, 101.03, 90.00 |
Refinement procedure
| Resolution | 47.960 - 1.570 |
| R-factor | 0.1532 |
| Rwork | 0.152 |
| R-free | 0.17540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.988 |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.960 | 1.590 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Number of reflections | 170135 | 5609 |
| <I/σ(I)> | 20.2 | |
| Completeness [%] | 95.0 | |
| Redundancy | 6.9 | |
| CC(1/2) | 1.000 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | 11% 1,5-pentadiol, 0.1 M BisTris/MOPSO pH 6.5, 7% PEG 8000. With 1:1200 trypsin |
