9GXZ

Structure of the Medicago truncatula LYR4 ectodomain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX IV BEAMLINE BioMAX
Synchrotron site	MAX IV
Beamline	BioMAX
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-11-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	32.247, 80.438, 46.898
Unit cell angles	90.00, 90.31, 90.00

Refinement procedure

Resolution	40.220 - 1.500
R-factor	0.1483
Rwork	0.146
R-free	0.18330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.016
RMSD bond angle	1.443
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.220	1.554
High resolution limit [Å]	1.500	1.500
Number of reflections	37799	3459
<I/σ(I)>	10.22
Completeness [%]	98.8
Redundancy	3.3
CC(1/2)	0.998	0.453

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	2M Ammonium sulfate, 0.1 M Bis-Tris buffer pH 5