9GX9
Crystal structure of CJO-1, a membrane-bound B1 metallo-beta-lactamase from Chryseobacterium joostei
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-01 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 1.27012 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 47.517, 68.156, 135.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.500 - 1.340 |
| R-factor | 0.1436 |
| Rwork | 0.142 |
| R-free | 0.17190 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.074 |
| Data reduction software | autoPROC |
| Data scaling software | STARANISO |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.502 | 1.456 |
| High resolution limit [Å] | 1.340 | 1.340 |
| Rpim | 0.019 | 0.356 |
| Number of reflections | 39335 | 1967 |
| <I/σ(I)> | 18.3 | 1.5 |
| Completeness [%] | 92.8 | 51.9 |
| Redundancy | 12.5 | 6.8 |
| CC(1/2) | 1.000 | 0.719 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 292.15 | 0.2 M MgCl2, 0.1 M Bis-Tris pH 5.5, 25% (w/v) PEG 3350 |
