9GVA
Crystal structure of the gamma carbonic anhydrase from Porphyromonas gingivalis
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 65 |
| Unit cell lengths | 120.752, 120.752, 91.310 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.370 - 2.400 |
| Rwork | 0.165 |
| R-free | 0.19650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.770 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.370 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.130 | 0.770 |
| Rmeas | 0.139 | 0.823 |
| Rpim | 0.049 | 0.289 |
| Number of reflections | 28525 | 3118 |
| <I/σ(I)> | 18.5 | 4.4 |
| Completeness [%] | 95.9 | 100 |
| Redundancy | 15.3 | 15.7 |
| CC(1/2) | 0.999 | 0.928 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 296 | 2.0 M ammonium sulfate, 0.1 M HEPES, pH 7.5, 2% v/v PEG 400 |
