9GUI
Crystal structure of transcription factor NtcA from Synechococcus elongatus in complex with its target DNA.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-09-06 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.9797 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 119.854, 46.935, 139.737 |
| Unit cell angles | 90.00, 98.62, 90.00 |
Refinement procedure
| Resolution | 24.170 - 3.000 |
| R-factor | 0.21585 |
| Rwork | 0.214 |
| R-free | 0.25250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.668 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.200 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 15466 | 15466 |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 98.5 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | NTCA-DNA complex was in 50 mM sodium citrate pH 6.5, 0.5 M NaCl, 5 mM magnesium cloride, 50 mM arginine hydrocloride, 50 mM Na L-glutamate and 10mM 2-oxoglutarate. CRYSTALLIZATION SOLUTION: 0,1M Bis-Tris, pH 6,5, 28% PEG monomethyl ether 2K. Cryo protectant: 38% PEG monomethyl ether |
