9GUH
Crystal structure of NtcA from S. elongatus in apo form B
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-10 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9762 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 96.385, 96.385, 126.893 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.190 - 3.330 |
R-factor | 0.22441 |
Rwork | 0.222 |
R-free | 0.27392 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.520 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 83.500 | 3.510 |
High resolution limit [Å] | 3.300 | 3.300 |
Number of reflections | 5530 | 5530 |
<I/σ(I)> | 4 | |
Completeness [%] | 100.0 | |
Redundancy | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | NtcA protein was at 6,1 mg/ml in 50 mM sodium citrate pH 6.5, 0.5 M NaCl, 5 mM magnesium cloride, 50 mM arginine hydrocloride, 50 mM Na L-glutamate. CRYSTALLIZATION SOLUTION: 0,05 M Tris-HCL pH8, 2M NaCl, 2% 2-methyl-2,4-pentanediol,10mM MgCl2, 5%DMSO. CRYO PROTECTANT: 27% 2-methyl-2,4-pentanediol |