9GU5
Crystal Structure of Hfq V22A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-08-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9737 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.182, 69.212, 73.193 |
| Unit cell angles | 63.54, 89.30, 60.04 |
Refinement procedure
| Resolution | 57.690 - 2.900 |
| R-factor | 0.2785 |
| Rwork | 0.274 |
| R-free | 0.38710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.979 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.20.1_4487: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 63.070 | 2.680 |
| High resolution limit [Å] | 2.640 | 2.640 |
| Rmerge | 0.231 | 2.319 |
| Number of reflections | 29610 | 1471 |
| <I/σ(I)> | 6 | |
| Completeness [%] | 98.8 | 96.5 |
| Redundancy | 3.6 | |
| CC(1/2) | 0.759 | 0.336 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 22.5 %v/v PEGSB, 0.2 M LiSO4, 0.05 M Zn Acet, 0.1 M BIS-TRIS |
