9GTI

X-ray crystal structure of mouse NPTN N-terminal domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-14
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9999
Spacegroup name	P 32 2 1
Unit cell lengths	85.907, 85.907, 163.285
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.930 - 2.030
R-factor	0.217
Rwork	0.215
R-free	0.24820
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.875
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER (AF-P97300-F1)
Refinement software	PHENIX (1.18.2-3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.930	2.100
High resolution limit [Å]	2.030	2.030
Rmerge	0.200
Rpim	0.047	0.630
Number of reflections	45798	4435
<I/σ(I)>	12	1.14
Completeness [%]	99.7	97.83
Redundancy	20
CC(1/2)	0.990	0.550

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.1 M HEPES pH 7.5, 30 % (v/v) PEG 400